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Updated: Jun 2, 2026

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
Published on: November 8, 2015
CYP3A5 genotype is not related to the intrapatient variability of tacrolimus clearance
Nilufar Pashaee1, Rachida Bouamar, Dennis A Hesselink
1Department of Hospital Pharmacy, Clinical Pharmacology Unit, Erasmus MC, Rotterdam, the Netherlands.
Background:
The risk of long-term chronic allograft nephropathy and graft loss after kidney transplantation is increased in patients with a high intrapatient variability of tacrolimus (Tac) clearance.
Methods:
To test whether this intrapatient variability is associated with an individual's CYP3A5 genotype, we measured the intrapatient variability in Tac clearance in a cohort of 208 kidney transplant recipients treated with Tac and mycophenolate mofetil.
Results:
Tac dose requirement was significantly higher in patients expressing CYP3A5. However, intraindividual variability of Tac clearance was not related to CYP3A5 genotype.
Conclusions:
Intraindividual variability in Tac clearance is not related to CYP3A5 genotype. Other factors, including patient adherence, may explain the variability in Tac clearance within an individual patient over time.
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