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Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
Published on: August 19, 2014
Nonrandom secondary chromosome-aberrations in liposarcomas with t(12, 16)
1UNIV LUND HOSP,DEPT ORTHOPED SURG,S-22185 LUND,SWEDEN. UNIV LUND HOSP,DEPT CLIN PATHOL,S-22185 LUND,SWEDEN. KAROLINSKA HOSP,DEPT ORTHOPED,TUMOR SERV,S-10401 STOCKHOLM 60,SWEDEN.
International Journal of Oncology
|May 14, 2011
Summary
Cytogenetic analysis of liposarcomas reveals a common t(12;16) translocation. Specific secondary aberrations, including isochromosome 7q, are uniquely associated with this translocation in liposarcoma tumors.
Area of Science:
- Cytogenetics
- Oncology
- Molecular Biology
Background:
- Liposarcoma is a malignant mesenchymal tumor.
- Recurrent chromosomal abnormalities are common in liposarcoma.
- The t(12;16)(q13;p11) translocation is a hallmark of myxoid liposarcoma.
Purpose of the Study:
- To cytogenetically analyze liposarcoma tumors.
- To identify recurrent chromosomal aberrations in liposarcoma.
- To compare aberration frequencies in tumors with and without the t(12;16) translocation.
Main Methods:
- Short-term culturing of ten liposarcoma tumors.
- Cytogenetic analysis including karyotyping.
- Review and comparison of published cytogenetic data for liposarcoma.
Main Results:
- Eight of ten liposarcomas exhibited the t(12;16)(q13;p11) translocation.
- Two tumors showed complex translocations involving chromosomes 7, 12, 16, and others.
- Isochromosome 7q (i(7q)) was observed in four tumors and is strongly associated with the t(12;16) aberration.
- Trisomy 5 and 8 were found in both groups, while +19, t(3;15), del(6q), i(7q), and rearrangements of 1p11 and 2q35 were specific to tumors with the 12;16 recombination.
Conclusions:
- The t(12;16)(q13;p11) translocation is a primary cytogenetic event in a subset of liposarcomas.
- Isochromosome 7q (i(7q)) represents a nonrandom secondary aberration specifically associated with the t(12;16) translocation.
- These findings refine the understanding of liposarcoma cytogenetics and may aid in diagnosis and classification.
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