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Related Concept Videos

Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...

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Related Experiment Video

Updated: Jun 2, 2026

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
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Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Published on: April 23, 2012

A rapid enzymatic method for aflatoxin B detection.

Danila Moscone1, Fabiana Arduini, Aziz Amine

  • 1Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Roma, Italy. danila.moscone@uniroma2.it

Methods in Molecular Biology (Clifton, N.J.)
|May 14, 2011
PubMed
Summary
This summary is machine-generated.

A novel method rapidly detects aflatoxin B (AFB) using acetylcholinesterase (AChE) inhibition. This cost-effective, reversible enzyme inhibition assay provides quick and easy screening for AFB in food products.

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Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds
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RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
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Published on: December 21, 2015

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RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem

Published on: December 21, 2015

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Food Safety

Background:

  • Aflatoxins, particularly aflatoxin B (AFB), are toxic secondary metabolites produced by Aspergillus fungi.
  • Contamination of food and feed with aflatoxins poses significant risks to human and animal health.
  • Accurate and rapid detection methods for AFB are crucial for food safety monitoring.

Purpose of the Study:

  • To develop and optimize a novel, rapid, and cost-effective method for aflatoxin B (AFB) determination.
  • To investigate the mechanism of AFB inhibition on acetylcholinesterase (AChE) activity.
  • To evaluate the method's suitability for AFB quantification in food matrices like barley.

Main Methods:

  • Utilized acetylcholinesterase (AChE) from electric eel as the enzyme indicator.
  • Employed Ellman's colorimetric method to measure residual AChE activity after AFB exposure.
  • Investigated AFB(1) inhibition kinetics, optimizing assay parameters (pH, time, temperature, substrate concentration).
  • Assessed cross-reactivity with other aflatoxins (AFB(2), AFG(1), AFG(2), AFM(1)) and validated in barley samples.

Main Results:

  • AFB(1) demonstrated reversible inhibition of AChE, independent of incubation time and enzyme concentration.
  • The optimized assay required only a 3-minute analysis time.
  • Established a linear detection range of 10-60 ng/mL for AFB(1).
  • Demonstrated good selectivity with minimal cross-reactivity with other tested aflatoxins.
  • Showed suitability for AFB(1) quantification in barley samples.

Conclusions:

  • A novel, rapid, and cost-effective method for AFB determination based on reversible AChE inhibition was successfully developed.
  • The assay's simplicity, speed, and cost-effectiveness make it suitable as a screening tool for aflatoxins.
  • This method offers a promising alternative for routine monitoring of AFB contamination in food and agricultural products.