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Wild-type Blocking PCR Combined with Sanger Sequencing for Detection of Low-frequency Somatic Mutation
Published on: August 23, 2024
PCR-RFLP to detect codon 248 mutation in exon 7 of p53 tumor suppressor gene
Liming Ouyang1, Chongtao Ge, Haizhen Wu
1Department of Applied biology, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
Abstract:
Individual genome DNA was extracted fast from oral swab and followed up with PCR specific for codon 248 of p53 tumor suppressor gene. MspI restriction mapping showed the G-C mutation in codon 248, which closely relates to cancer susceptibility. Students learn the concepts, detection techniques, and research significance of point mutations or single nucleotide polymorphisms in individual genomes.
