Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

High-Performance Liquid Chromatography: Introduction

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

PRMT6 acts as a pro-angiogenic factor in colorectal cancer.

Cell death discovery·2026

Same author

<b>Erratum: HAIFENG HAN, XIAOJIN XUE, ZHISHENG ZHANG & CHEN SHAO (2026) Morphology and molecular phylogeny of a new ciliate, <i>Hemiurosomoida sinica</i> sp. nov. (Ciliophora, Hypotrichia). <i>Zootaxa</i>, 5750 (2): 241-250.</b>

Zootaxa·2026

Same author

<b>Morphology and molecular phylogeny of a new ciliate, <i>Hemiurosomoida sinica</i> sp. nov. (Ciliophora, Hypotrichia)</b>.

Zootaxa·2026

Same author

Lactic acid promotes metastasis of papillary thyroid carcinoma by enhancing CPT1A lactylation.

Cell death & disease·2026

Same author

Learning Curve and Generalizability for Modified Open Anterior Mesh Hernia Repair.

JAMA surgery·2025

Same author

Morphology of a novel ciliate, <i>Oxytrichachongqingica</i> sp. nov. (Ciliophora, Hypotrichia).

ZooKeys·2025

Search research articles

Related Experiment Video

Updated: Jun 2, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Polymeric ionic liquid-coated capillary for capillary electrophoresis.

Jing Li¹, Haifeng Han, Qing Wang

¹Key Laboratory of Chemistry of Northwestern Plant Resources, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, PR China.

Journal of Separation Science

|May 14, 2011

Summary

A new polymeric ionic liquid (PIL) coating for capillary electrophoresis (CE) offers reversed electroosmotic flow and good tolerance. This effective PIL coating enables fast separation of proteins and anions, including organic acids in grape juice.

More Related Videos

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Published on: October 26, 2016

Related Experiment Videos

Last Updated: Jun 2, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Published on: October 26, 2016

Area of Science:

Analytical Chemistry
Separation Science
Polymer Chemistry

Background:

Capillary electrophoresis (CE) is a powerful separation technique.
Developing novel coatings for CE capillaries is crucial for enhanced performance.
Polymeric ionic liquids (PILs) offer unique properties for surface modification.

Purpose of the Study:

To report a new application of polymeric ionic liquid (PIL) in capillary electrophoresis (CE).
To investigate the properties of a PIL-coated capillary for CE analysis.
To evaluate the separation capabilities of the PIL-coated capillary for various analytes.

Main Methods:

Physical adsorption of poly(1-vinyl-3-butylimidazolium bromide) on a silica capillary.
Characterization of electroosmotic flow (EOF) dependence on pH for bare and PIL-coated capillaries.
Testing the tolerance of the coated capillary to organic solvents, NaOH, and HCl.
Application of the PIL-coated capillary for separating basic proteins, anionic analytes, and organic acids in grape juice.

Main Results:

The PIL-coated capillary exhibited reversed electroosmotic flow (EOF) over a wide pH range (3.0-9.0) with good repeatability.
The coated capillary demonstrated good tolerance to organic solvents, 0.1 M NaOH, and 0.1 M HCl.
Successful separation of basic proteins and anionic analytes was achieved.
Organic acid additives in grape juice were effectively separated using the PIL-coated capillary.

Conclusions:

Polymeric ionic liquid (PIL) coating provides a simple and effective method for capillary electrophoresis (CE) capillary modification.
The PIL-coated capillary offers an alternative for the separation of anions and basic proteins.
This novel coating enhances CE performance and expands its applicability in complex sample analysis.