Related Experiment Video
Updated: Jun 2, 2026
08:07
Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
Published on: August 2, 2015
Interaction between MAK-V protein kinase and synaptopodin
S V Kalinichenko1, P N Vikhreva, I V Korobko
1Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia. skalin@rambler.ru
Biochemistry. Biokhimiia
|May 17, 2011
Summary
MAK-V protein kinase interacts with synaptopodin, a protein crucial for dendritic spine formation and synaptic plasticity. This discovery sheds light on MAK-V
Area of Science:
- Neuroscience
- Molecular Biology
- Cell Biology
Background:
- MAK-V protein kinase (HUNK) functions remain largely uncharacterized despite its discovery over a decade ago.
- Understanding MAK-V's molecular mechanisms is essential for elucidating its cellular roles.
Purpose of the Study:
- To identify proteins interacting with the C-terminal domain of MAK-V protein kinase.
- To investigate the functional implications of MAK-V interactions in the nervous system.
Main Methods:
- Protein-protein interaction studies focusing on the C-terminal domain of MAK-V.
- Confirmation of identified interactions using various experimental approaches.
Main Results:
- Synaptopodin was identified as a novel interaction partner for MAK-V protein kinase.
- The interaction between MAK-V and synaptopodin was experimentally validated.
Conclusions:
- The interaction between MAK-V and synaptopodin suggests a role for MAK-V in cellular processes involving synaptopodin, such as dendritic spine formation and synaptic plasticity.
- This finding opens new avenues for research into MAK-V's function in the nervous system.
Related Concept Videos
01:25cAMP-dependent Protein Kinase Pathways
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
01:42Microtubule Associated Proteins (MAPs)
Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
02:54Protein Kinases and Phosphatases
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
02:54Protein Kinases and Phosphatases
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
01:09Cytoskeletal Linker Proteins - Plakins
Plakins are large proteins with binding domains for microtubules, microfilaments, intermediate filaments, and membrane-associated protein complexes at cell junctions. Plakin functions are evolutionarily conserved and are primarily involved in organizing the different components of the cytoskeleton by crosslinking them to each other and connecting them to the cell-matrix and cell adhesion complexes. They are also known to interact with signal transducers, serve as scaffolds for signaling...
01:20The JAK-STAT Signaling Pathway
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...