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miRNA Expression Analyses in Prostate Cancer Clinical Tissues
Published on: September 8, 2015
5-Lipoxygenase expression in benign and malignant canine prostate tissues
L A Goodman1, C L Jarrett, T M Krunkosky
1Department of Small Animal Medicine and Surgery, University of Georgia College of Veterinary Medicine, Athens, GA 30602, USA.
Veterinary and Comparative Oncology
|May 17, 2011
Summary
5-Lipoxygenase (5-LO) is present in canine prostate tissues. Its expression decreases in metastatic canine prostate cancer, suggesting a role in disease progression.
Area of Science:
- Veterinary Oncology
- Molecular Biology
- Biochemistry
Background:
- 5-Lipoxygenase (5-LO) is overexpressed in human prostate cancer, inhibiting proliferation and inducing apoptosis.
- The role of 5-LO in canine prostate cancer (PC) pathogenesis is not well understood.
Purpose of the Study:
- To investigate 5-LO expression in canine PC compared to benign prostate tissue.
- To determine if 5-LO expression changes with the progression of canine PC.
Main Methods:
- Immunoblot analysis of canine PC and benign prostatic hyperplasia (BPH) tissues.
- Immunohistochemical staining for 5-LO in primary PC, BPH, and suppurative prostatitis.
- Quantification of 5-LO expressing tumor cells in primary and metastatic PC lesions.
Main Results:
- 5-LO was expressed in both canine PC and BPH tissues.
- No significant difference in 5-LO staining was observed in stromal or epithelial components across primary PC, BPH, or prostatitis.
- Metastatic PC lesions showed significantly lower tumor cell expression of 5-LO compared to primary PC.
Conclusions:
- Differential expression of 5-LO does not appear to occur in primary canine prostate conditions (PC, BPH, prostatitis).
- Decreased 5-LO expression in metastatic lesions suggests enzyme down-regulation or altered expression during canine PC progression to metastasis.
