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Stereoscopic observation of enzyme distribution in lowicryl K4M-embedded specimens by conventional electron
K Tomiyasu1, F Nasu, H Igarashi
12nd Department of Anatomy, Toho University School of Medicine, Tokyo, Japan.
Abstract:
The present investigation was undertaken to explore the value of the Lowicryl K4M embedding technique for enzyme histochemical examination of semi-thin sections. The low-temperature embedding procedure with Lowicryl K4M was found to provide favorable conditions for preservation of enzyme activity in tissue samples. We tested the histological effects of various fixatives; the best results were obtained using 4% paraformaldehyde when testing for AcPase, AlPase, TPPase, and Mg-ATPase in the dorsal root ganglion. The three-dimensional cellular fine structure could be clearly seen in stereo pair pictures under stereoscopy.