Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Karyotyping

Karyotyping

Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...

Lampbrush Chromosomes

Lampbrush Chromosomes

In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops resemble the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Scapular belt for the treatment of comminuted fractures of scapula].

Zhongguo gu shang = China journal of orthopaedics and traumatology·2010

Same author

Manipulation of ordered nanostructures of protonated polyoxometalate through covalently bonded modification.

Chemistry (Weinheim an der Bergstrasse, Germany)·2010

Same author

Developments in nonsteroidal antiandrogens targeting the androgen receptor.

ChemMedChem·2010

Same author

Dynamic presentation of immobilized ligands regulated through biomolecular recognition.

Journal of the American Chemical Society·2010

Same author

[Research on crop-weed discrimination using a field imaging spectrometer].

Guang pu xue yu guang pu fen xi = Guang pu·2010

Same author

A palladium/copper bimetallic catalytic system: dramatic improvement for Suzuki-Miyaura-type direct C-H arylation of azoles with arylboronic acids.

Chemistry (Weinheim an der Bergstrasse, Germany)·2010

Search research articles

Related Experiment Video

Updated: Jun 2, 2026

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

Published on: December 16, 2015

A sequence-based genetic linkage map as a reference for Brassica rapa pseudochromosome assembly.

Yan Wang¹, Silong Sun, Bo Liu

¹Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

|May 17, 2011

Summary

A new genetic map for Brassica rapa was created using 119 doubled haploid lines and 507 markers. This map anchors 82.9% of the genome, improving pseudochromosome assembly and aiding Brassica research.

More Related Videos

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

Published on: June 28, 2012

Related Experiment Videos

Last Updated: Jun 2, 2026

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

Published on: December 16, 2015

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

Published on: June 28, 2012

Area of Science:

Plant genetics
Genomics
Crop science

Background:

Brassica rapa is a vital crop and model organism for polyploidization studies.
Previous genome sequencing efforts had incomplete pseudochromosome assembly, anchoring only 73.6% of scaffolds.
A more comprehensive genetic map was needed to improve genome assembly.

Purpose of the Study:

To construct a reference genetic linkage map for Brassica rapa.
To facilitate the anchoring of sequence scaffolds from genome sequencing efforts.
To enhance the assembly of Brassica rapa pseudochromosomes.

Main Methods:

Developed PCR-based insertion/deletion (InDel) markers by re-sequencing parental lines.
Utilized 119 doubled haploid (DH) lines from a specific cross.
Constructed a linkage map comprising 415 InDels and 92 SSRs, totaling 507 markers.

Main Results:

Identified ten linkage groups (A01-A10) with a total map length of 1234.2 cM.
Anchored 152 scaffolds to chromosomes, covering 82.9% of the Brassica rapa genome.
Combined with existing maps, achieved 88.9% genome coverage with 183 anchored scaffolds.

Conclusions:

The developed linkage map is crucial for integrating genome sequences and genetic information.
It serves as a valuable resource for the international Brassica research community.
This map significantly advances the pseudochromosome assembly of the Brassica rapa genome.