Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Na current in membrane blebs: implications for channel mobility and patch clamp recording.

R L Milton1, J H Caldwell

  • 1Department of Cellular and Structural Biology, University of Colorado Health Sciences Center, Denver 80262.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|March 1, 1990
PubMed
Summary

Suction-induced membrane blebs in muscle fibers contain sodium channels, complicating voltage clamp measurements. These blebs, enriched in lipids, have a lower sodium channel density than normal surface membrane.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cardiac receptor physiology and its application to clinical imaging: present and future.

Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology·2001
Same author

Compact myelin dictates the differential targeting of two sodium channel isoforms in the same axon.

Neuron·2001
Same author

Right ventricular involvement in hypertrophic cardiomyopathy: a case report and literature review.

Clinical cardiology·2001
Same author

Nomenclature of voltage-gated sodium channels.

Neuron·2001
Same author

GOLAC: an endogenous anion channel of the Golgi complex.

Biophysical journal·2000
Same author

Sodium channel Na(v)1.6 is localized at nodes of ranvier, dendrites, and synapses.

Proceedings of the National Academy of Sciences of the United States of America·2000

Area of Science:

  • Cell biology
  • Biophysics
  • Neuroscience

Background:

  • Loose patch clamp recordings are used to measure ion channel activity in muscle fibers.
  • Suction applied during these recordings can induce the formation of membrane blebs.
  • The presence of ion channels within these blebs could interfere with accurate electrophysiological measurements.

Purpose of the Study:

  • To investigate the properties of suction-induced membrane blebs in enzymatically dissociated muscle fibers.
  • To determine the ion channel content, specifically sodium channels, within these blebs.
  • To assess the impact of bleb formation on loose patch voltage clamp measurements.

Main Methods:

  • Enzymatically dissociated muscle fibers were subjected to suction while recording with loose patch pipettes.

Related Experiment Videos

  • Membrane capacitance and sodium current were measured before and after bleb formation.
  • Sodium current and capacitance were normalized to assess protein and lipid content.

    • Main Results:

    • Suction-induced blebs increased membrane capacitance and sodium current.
    • Ejecting blebs returned capacitance to baseline but left sodium current reduced.
    • Bleb membrane exhibited a sodium channel density approximately 50% lower than normal surface membrane.

    Conclusions:

    • Suction-induced blebs are enriched in membrane lipids and relatively depleted of proteins, including sodium channels.
    • Bleb formation alters the local membrane composition, affecting the accuracy of electrophysiological studies.
    • These findings highlight the importance of considering bleb formation artifacts in patch clamp experiments.