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Updated: Jun 2, 2026
In Vitro Assay to Study Tumor-macrophage Interaction
Published on: August 1, 2019
[Study of the antitumor activity of alveolar macrophages after transfected human INF-γ gene]
Fengli Zhou1, Xiaogang Bi, Tiantuo Zhang
1Department of Respiratory Medicine, the Third Afiliated Hospital, Zhongshan University, Guangzhou 510630, China. zhoufenglizfl@126.com
Background And Objective:
Alveolar macrophages (AMs) activated have the antitumor activity. The interferon-γ (INF-γ) is one of the stimulating factors. INF-γ can enhance the immune function of AMs in vitro. The aim of this study is to investigate the effect of human INF-γ gene on the antitumor activity of AMs when transfected into the alveolar macrophages (AMs) from the patients with lung cancer in vitro.
Methods:
AMs obtained by brochoalveolar lavage were separated and transfected by INF-γ gene. RT-PCR and ELISA were applied to determine whether the transfection was successful. The levels of tumor necrosis factor α (TNF-α), nitric oxide (NO) and interleukin-1 (IL-1) produced by AMs and the killing activity of AMs against L1210 cells was detected respectively.
Results:
Both RT-PCR and ELISA demonstrated that human INF-γ gene had been successfully transfected into AMs. When transfected by human INF-γ gene, the levels of TNF-α, NO and IL-1 produced by AMs from the patients with lung cancer and the killing activity of AMs against L1210 cells were significantly higher than those of the control groups.
Conclusion:
Human INF-γ gene can enhance the antitumor activity of AMs when transfected into AMs from the patients with lung cancer.
