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Related Experiment Video

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Dissection of Larval Zebrafish Gonadal Tissue
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Expression patterns of lgr4 and lgr6 during zebrafish development.

Kentaro Hirose1, Nobuyoshi Shimoda, Yutaka Kikuchi

  • 1Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Japan.

Gene Expression Patterns : GEP
|May 17, 2011
PubMed
Summary

This study characterizes the embryonic and larval expression patterns of zebrafish Lgr4 and Lgr6, key G protein-coupled receptors involved in organ development and cell proliferation. The findings provide a foundation for future research into the functions of these zebrafish Lgr genes.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Leucine-rich repeat (LRR)-containing G protein-coupled receptors (LGRs) are crucial for development.
  • Mammalian Lgr4, Lgr5, and Lgr6 have known roles in cell proliferation and as stem cell markers.
  • Expression profiles of zebrafish Lgr4 and Lgr6 during embryonic and larval stages were previously undefined.

Purpose of the Study:

  • To clone and characterize two zebrafish Lgr genes, orthologous to mammalian Lgr4 and Lgr6.
  • To define the spatiotemporal expression patterns of zebrafish lgr4 and lgr6 during embryonic and larval development.
  • To provide a foundation for future functional studies of Lgr4 and Lgr6 in zebrafish organogenesis.

Main Methods:

  • Cloning of zebrafish lgr4 and lgr6 genes using zebrafish genomic database.

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Last Updated: Jun 2, 2026

Dissection of Larval Zebrafish Gonadal Tissue
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Dissection of Larval Zebrafish Gonadal Tissue

Published on: April 26, 2017

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
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High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

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05:36

Dissection and Lateral Mounting of Zebrafish Embryos: Analysis of Spinal Cord Development

Published on: February 28, 2014

  • Phylogenetic analysis to confirm orthology with mammalian Lgr4 and Lgr6.
  • Whole-mount in situ hybridization to determine gene expression patterns at various developmental time points (24-96 hours post-fertilization).

    • Main Results:

    • Zebrafish lgr4 expression was observed in the neural plate border, otic vesicles, midbrain, eyes, and digestive organs.
    • Zebrafish lgr6 expression was detected in the notochord, Kupffer's vesicle, otic vesicles, and lateral line primordia.
    • Expression patterns for both genes were detailed across multiple developmental stages, highlighting their involvement in diverse developing tissues.

    Conclusions:

    • The study successfully identified and characterized zebrafish Lgr4 and Lgr6 orthologs.
    • Detailed expression maps of lgr4 and lgr6 during zebrafish development were established.
    • These findings are essential for future investigations into the roles of Lgr4 and Lgr6 in zebrafish cell differentiation and organ development.