One plate, two plates, a thousand plates. How crystallisation changes with large numbers of samples

Area of Science:

• Biochemistry
• Structural Biology
• Laboratory Automation

Background:

• The Collaborative Crystallisation Centre (C3) aims to provide high-throughput protein crystallization services.
• Achieving high-throughput requires more than just rapid experiment setup; it demands sustainable, efficient processes.

Purpose of the Study:

• To describe the protocols and lessons learned in establishing and operating a high-throughput crystallization center.
• To highlight the importance of process implementation, quality control, and communication in large-scale lab automation.

Main Methods:

• Development and implementation of enabling protocols for protein sample handling and crystallization.
• Integration of quality control measures, including evaporation checks and sample tracking systems.
• Focus on establishing effective communication channels between the center and its clients, and among staff.

Main Results:

• Successful setup of approximately two thousand 96-well plates annually for crystallization screening and optimization.
• Implementation of various protocols, from basic storage checks to advanced sample labeling and tracking.
• Identification of effective communication as a critical, often underestimated, factor in high-throughput operations.

Conclusions:

• Sustainable high-throughput operations depend on well-defined processes, rigorous checks, and efficient automation.
• Effective communication is paramount for managing client expectations and internal workflow in a high-throughput setting.
• The C3's experience demonstrates the practical challenges and solutions in scaling lab automation for structural biology research.