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Updated: Jun 2, 2026
06:34
Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Blautia stercoris sp. nov., isolated from human faeces
Seong-Kyu Park1, Min-Soo Kim1, Seong Woon Roh1
1Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Summary
A novel bacterium, Blautia stercoris sp. nov., was identified from human feces. This strictly anaerobic, Gram-positive bacterium offers new insights into the Lachnospiraceae family
Area of Science:
- Microbiology
- Bacteriology
- Genomics
Background:
- The human gut microbiome harbors diverse bacterial species.
- Understanding novel bacterial isolates is crucial for microbiome research.
Purpose of the Study:
- To characterize and classify a newly isolated bacterial strain from human feces.
- To determine the phylogenetic and phenotypic properties of the novel strain.
Main Methods:
- Phylogenetic analysis using 16S rRNA gene sequences.
- Phenotypic characterization including biochemical tests and fatty acid analysis.
- Genomic DNA G+C content determination.
Main Results:
- Strain GAM6-1T, a novel Gram-positive, anaerobic bacterium, was isolated.
- Phylogenetic analysis placed it within the Lachnospiraceae family, closely related to Blautia producta.
- Characterization revealed specific fatty acid profiles and acid production from carbohydrates, with acetic acid as a major fermentation product.
- Genomic DNA G+C content was determined to be 35.6 mol%.
Conclusions:
- Strain GAM6-1T represents a new species, Blautia stercoris sp. nov.
- This finding expands the known diversity within the Blautia genus.
- The study provides a comprehensive description of a novel human gut bacterium.
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