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Updated: Jun 2, 2026
Isolation and Culturing of Primary Murine Adipocytes from Lean and Obese Mice
Published on: January 24, 2025
Prieurianin Causes Weight Loss in Diet-Induced Obese Mice and Inhibits Adipogenesis in Cultured Preadipocytes
Ahmed Kablan1, Rudel A Saunders, Maria Szkudlarek-Mikho
1Department of Medicine, University of Toledo, College of Medicine, Toledo, OH, United States.
Abstract:
The global increase in the incidence of obesity has emerged as one of the most serious public health risks in recent years. Despite the enormity of the obesity pandemic, there are currently only two FDA-approved therapies for its treatment and these drugs exhibit modest efficacy and have limiting side effects. Prieurianin is a plant limonoid product that deters feeding in insect larvae. We investigated in this study the effects of prieurianin on weight loss and adipogenesis. Our results showed that prieurianin causes weight loss by reducing energy intake in obese mice on high-calorie diet. We also found that prieurianin is anti-adipogenic in cultured preadipocytes and adipocytes by inhibiting proliferation and differentiation of preadipocytes into adipocytes, and induces either dedifferentiation or delipidation of mature adipocytes. Whether prieurianin can potentially be used for obesity treatment in human warrants further investigation.
Insights
Prieurianin, a plant compound, effectively reduces calorie intake and promotes weight loss in obese mice. It also inhibits fat cell formation and may offer a new avenue for obesity treatment.
Area of Science:
- Pharmacology
- Metabolic Diseases
- Natural Products
Background:
- Obesity is a global health crisis with limited effective treatments.
- Current FDA-approved obesity therapies have modest efficacy and side effects.
Purpose of the Study:
- To investigate the effects of prieurianin on weight loss and adipogenesis.
- To evaluate prieurianin as a potential therapeutic agent for obesity.
Main Methods:
- Administered prieurianin to obese mice on a high-calorie diet.
- Assessed prieurianin's impact on energy intake and body weight.
- Examined prieurianin's effects on preadipocyte proliferation, differentiation, and mature adipocyte function in vitro.
Main Results:
- Prieurianin significantly reduced energy intake, leading to weight loss in obese mice.
- Prieurianin demonstrated anti-adipogenic properties by inhibiting preadipocyte proliferation and differentiation.
- Prieurianin induced dedifferentiation or delipidation in mature adipocytes.
Conclusions:
- Prieurianin exhibits potential as an anti-obesity agent by reducing food intake and inhibiting fat cell development.
- Further research is warranted to explore prieurianin's efficacy and safety for human obesity treatment.
