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Updated: Jun 2, 2026

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Published on: November 7, 2019

Multizone paper platform for 3D cell cultures.

Ratmir Derda¹, Sindy K Y Tang, Anna Laromaine

¹Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, United States of America.

|May 17, 2011

Summary

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This study introduces a simple method for creating and analyzing 3D tissue cultures. The "cells-in-gels-in-paper" (CiGiP) technique allows for easy isolation of specific cell layers for high-throughput analysis.

Area of Science:

Biotechnology
Tissue Engineering
Cell Biology

Background:

In vitro 3D cultures mimic in vivo tissues but lack regional analysis.
Current methods for isolating cells from 3D cultures are low-throughput.
Physiological differences in 3D cultures arise from environmental gradients.

Purpose of the Study:

To develop a simple, high-throughput method for culturing and analyzing 3D tissue models.
To enable the isolation of cells from specific regions within 3D constructs.
To facilitate studies on spatial composition and cell migration in 3D models.

Main Methods:

Utilized patterned paper with hydrophilic zones for cell culture.
Created 200-micron-thick extracellular matrix (ECM) gel slabs containing cells.

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Published on: December 26, 2010

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Last Updated: Jun 2, 2026

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

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3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

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Published on: December 26, 2010

Assembled 96 3D multilayer constructs by stacking paper layers.

Developed a de-stacking method for simultaneous sectioning of all cultures.

Main Results:

Successfully cultured arrays of thin planar tissue sections.
Enabled simple isolation of 200-micron-thick cell-containing slabs.
Demonstrated control over spatial cell distribution in stacked 3D cultures.
Facilitated comparative growth studies of 3D tumor models with varying spatial composition.

Conclusions:

The CiGiP method provides a simple, high-throughput approach for 3D culture and analysis.
This technique allows for regional cell isolation and examination of spatial dynamics.
Offers a valuable tool for studying complex 3D tissue models, including tumor microenvironments.