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Related Concept Videos

Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...

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A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
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Gene induction by interferons in bt-20 cells.

R Tiwari, L Guo, B Mukhopadhyay

    International Journal of Oncology
    |May 17, 2011
    PubMed
    Summary
    This summary is machine-generated.

    Interferons (IFNs) exhibit anti-proliferative effects on cancer cells. Cell type, particularly estrogen receptor status, influences how IFNs affect gene expression and biological outcomes.

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    High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
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    Published on: March 24, 2015

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Immunology

    Background:

    • Interferons (IFNs) possess anti-proliferative properties against diverse cell lines.
    • The precise molecular mechanisms underlying IFN effects are linked to IFN-inducible genes.
    • Previous research demonstrated IFN-induced anti-proliferation and gene induction in MCF-7 cells.

    Purpose of the Study:

    • To investigate the impact of type I and II interferons on the estrogen receptor-negative BT-20 cell line.
    • To compare IFN-induced gene expression patterns between BT-20 and MCF-7 cells.
    • To elucidate the role of cell-specific characteristics, including estrogen receptor presence, in IFN responsiveness.

    Main Methods:

    • Treatment of BT-20 cells with type I and II interferons.
    • Analysis of IFN-inducible gene expression.
    • Comparison of gene induction kinetics and RNA splicing patterns between BT-20 and MCF-7 cell lines.

    Main Results:

    • Interferons demonstrated anti-proliferative effects on BT-20 cells.
    • Several IFN-inducible genes were activated in BT-20 cells.
    • Differences in gene induction and RNA splicing were observed compared to MCF-7 cells, notably mRNA 561 induction by IFN-gamma and differential splicing of mRNA 6-16.

    Conclusions:

    • The biological effects of interferons are significantly influenced by specific cellular characteristics.
    • Estrogen receptor status in cell lines can modulate the expression of interferon-responsive mRNAs.
    • Cell-specific responses highlight the complexity of interferon-mediated anti-cancer effects.