Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biological characterization of nef in long-term survivors of human immunodeficiency virus type 1 infection.

Journal of virology·1995
Same author

Virulence characteristics of Escherichia coli causing first urinary tract infection predict risk of second infection.

The Journal of infectious diseases·1995
Same author

Concordant loss of imprinting of the human insulin-like growth factor II gene promoters in cancer.

The Journal of biological chemistry·1995
Same author

Altered cAMP levels in retinas from transgenic mice expressing a rhodopsin mutant.

Biochemical and biophysical research communications·1995
Same author

Repetitive Ser-Gly sequences enhance heparan sulfate assembly in proteoglycans.

The Journal of biological chemistry·1995
Same author

The angiotensin II type 2 (AT2) receptor antagonizes the growth effects of the AT1 receptor: gain-of-function study using gene transfer.

Proceedings of the National Academy of Sciences of the United States of America·1995

Related Experiment Video

Updated: Jun 2, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

P53 expression during multistage human oral carcinogenesis.

L Zhang1, M Rosin, R Priddy

  • 1BC CANC RES CTR,VANCOUVER V5Z 4E6,BC,CANADA.

International Journal of Oncology
|May 17, 2011
PubMed
Summary

p53 protein expression increases with oral dysplasia and squamous cell carcinoma (SCC) severity. This suggests p53 mutation is an early oral cancer event and p53 may serve as an early marker for high-risk lesions.

More Related Videos

Identification of OTX1 and OTX2 As Two Possible Molecular Markers for Sinonasal Carcinomas and Olfactory Neuroblastomas
07:00

Identification of OTX1 and OTX2 As Two Possible Molecular Markers for Sinonasal Carcinomas and Olfactory Neuroblastomas

Published on: February 28, 2019

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
04:56

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

Related Experiment Videos

Last Updated: Jun 2, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Identification of OTX1 and OTX2 As Two Possible Molecular Markers for Sinonasal Carcinomas and Olfactory Neuroblastomas
07:00

Identification of OTX1 and OTX2 As Two Possible Molecular Markers for Sinonasal Carcinomas and Olfactory Neuroblastomas

Published on: February 28, 2019

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
04:56

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

Area of Science:

  • Oncology
  • Oral Pathology
  • Molecular Biology

Background:

  • Oral premalignant lesions and squamous cell carcinomas (SCC) represent a spectrum of oral cancer development.
  • Understanding molecular markers associated with malignant transformation is crucial for early detection and intervention.

Purpose of the Study:

  • To investigate the immunohistochemical expression of p53 in oral premalignancies and SCC.
  • To correlate p53 expression levels with the degree of dysplasia in oral lesions.

Main Methods:

  • Immunohistochemical analysis of p53 protein expression was performed on oral premalignant lesions (including mild dysplasia), oral SCC, and hyperplastic oral lesions.
  • Quantitative and qualitative assessment of p53 staining intensity and cellular distribution was conducted.

Main Results:

  • p53 staining was detected in 56% of oral premalignant lesions and 70% of oral SCC, but absent in hyperplastic lesions.
  • A significant increase in p53 staining percentage, intensity, and cellular expansion was observed with increasing degrees of dysplasia and in SCC.
  • These findings indicate a progressive accumulation of p53 protein during oral carcinogenesis.

Conclusions:

  • p53 gene mutation appears to be an early event in the development of oral cancer.
  • p53 protein expression can serve as an early adjunctive marker for identifying oral premalignant lesions with a higher potential for malignant transformation.