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Updated: Jun 2, 2026
09:25
Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
Published on: October 14, 2016
Sequential and progressive cyclin expression in human osteosarcoma cells - diagnostic and therapeutic implications
L Wu1, R Williams, D Carbonarohall
1UNIV SO CALIF,CHILDRENS HOSP LOS ANGELES,SCH MED,DIV ORTHOPAED SURG,4650 SUNSET BLVD,LOS ANGELES,CA 90027. UNIV SO CALIF,SCH PHARM,DEPT MOLEC PHARMACOL & TOXICOL,LOS ANGELES,CA 90027.
International Journal of Oncology
|May 17, 2011
Summary
Cyclin D1 is crucial for osteosarcoma cell proliferation and is overexpressed in Ewing
Area of Science:
- Cell Biology
- Molecular Biology
- Cancer Research
Background:
- Cyclins and cyclin-dependent kinases (Cdks) regulate cell cycle transitions.
- Dysregulation of these enzymes is implicated in human tumorigenesis.
Purpose of the Study:
- To characterize the expression of cyclins D1, E, A, and B in MG-63 osteosarcoma cells.
- To investigate the role of cyclin D1 in osteosarcoma cell proliferation.
- To examine cyclin D1 amplification and overexpression in Ewing's sarcoma.
Main Methods:
- Cell synchronization and successive cyclin expression analysis.
- Antisense oligo-deoxynucleotide strategy to inhibit cyclin D1.
- Comparative analysis of cyclin D1 in osteosarcoma and Ewing's sarcoma cell lines.
Main Results:
- Sequential expression of cyclins D1, E, A, and B was observed.
- Cyclin D1 demonstrated essential role in osteosarcoma cell proliferation.
- Cyclin D1 amplification and overexpression were found in Ewing's sarcoma.
Conclusions:
- Findings support a sequential model of cyclin expression.
- Cyclin D1 plays a significant role in osteosarcoma and Ewing's sarcoma.
- Cyclin levels may serve as diagnostic/prognostic markers for proliferative disorders.
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