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Ras gene activation in human small intestinal tumors.

D Spandidos1, T Liloglou, D Arvanitis

  • 1UNIV CRETE,SCH MED,IRAKLION,GREECE. AGHIA SOPHIA CHILDRENS HOSP,ATHENS,GREECE. AIR FORCES GEN & VA HOSP,ATHENS,GREECE.

International Journal of Oncology
|May 17, 2011
PubMed
Summary
This summary is machine-generated.

Ras p21 oncoprotein was elevated in most small intestinal tumors. H-ras gene mutations were detected for the first time in these tumors, suggesting a role in pathogenesis.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Gastroenterology

Background:

  • The ras oncoprotein plays a role in cell signaling and cancer development.
  • Investigating ras p21 expression and mutations in small intestinal tumors is crucial for understanding their pathogenesis.

Purpose of the Study:

  • To investigate the expression of ras p21 oncoprotein in human small intestinal tumors.
  • To detect H-ras and K-ras mutations in codon 12 and 13 in these tumors.
  • To explore the potential role of ras p21 and H-ras mutations as molecular markers in small intestinal tumors.

Main Methods:

  • Immunohistochemical technique using monoclonal antibody Y13-259 to analyze ras p21 levels.
  • Polymerase chain reaction (PCR) to detect H-ras and K-ras mutations.
  • Comparison of protein levels and mutation status between tumor tissues and adjacent normal tissues.

Main Results:

  • Elevated ras p21 oncoprotein levels were observed in 9 out of 13 small intestinal tumors compared to normal tissue.
  • H-ras gene mutations in codon 12 were identified in 4 out of 13 tumors, including a Brunner's gland adenoma, lymphoma, leiomyosarcoma, and metastatic adenocarcinoma.
  • No K-ras mutations were found in codons 12 or 13.
  • This study represents the first demonstration of ras mutations in small intestinal tumors.

Conclusions:

  • Ras p21 oncoprotein may be involved in the pathogenesis of small intestinal tumors.
  • H-ras mutations are present in a subset of small intestinal tumors.
  • Ras p21 oncoprotein and H-ras mutations may serve as molecular genetic markers for small intestinal tumors.