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Updated: Jun 2, 2026
A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma
Published on: September 30, 2021
Growth-inhibition and change in morphology and motility of sclc cell-lines by hepatocyte growth-factor scatter factor
K Rygaard1, B Klausen, T Nakamura
1KYUSHU UNIV,FAC SCI,DEPT BIOL,FUKUOKA 812,JAPAN.
Abstract:
Expression of the c-met proto-oncogene is common iii small cell lung cancer (SCLC) cell lines. The product of this proto-oncogene is the receptor for hepatocyte growth factor (HGF), also known as scatter factor (SF) and hepatopoietin A. We examined the effect of HGF/SF on 8 SCLC cell lines of which six expressed c-Met and 2 control cell lines did not. The effect was monitored by growth curves, in vitro migration in Boyden chambers. and by examination of morphology. Three cell lines responded in one or more of the following ways: Growth inhibition, morphological alterations. and increased migration in Boyden chambers. We conclude that functional c-Met receptors are frequently expressed in SCLC and may contribute to the behaviour of this tumour type.
Insights
The c-Met proto-oncogene is frequently expressed in small cell lung cancer (SCLC) cell lines. Its receptor, HGF/SF, influences SCLC cell behavior, including growth, morphology, and migration.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- The c-met proto-oncogene is often expressed in small cell lung cancer (SCLC).
- c-Met is the receptor for hepatocyte growth factor (HGF), also known as scatter factor (SF).
Purpose of the Study:
- To investigate the functional role of HGF/SF signaling in SCLC.
- To determine if c-Met receptor expression correlates with SCLC cell behavior.
Main Methods:
- Assessed c-Met expression in 8 SCLC cell lines.
- Applied HGF/SF to cell lines and monitored effects on growth curves, in vitro migration (Boyden chambers), and cell morphology.
Main Results:
- Six of eight SCLC cell lines expressed c-Met.
- Three cell lines exhibited growth inhibition, morphological alterations, and/or increased migration in response to HGF/SF.
Conclusions:
- Functional c-Met receptors are common in SCLC.
- HGF/SF signaling may play a significant role in the behavior and progression of SCLC.
