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Updated: Sep 24, 2026

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
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Published on: August 18, 2012

Subcellular distribution of a nitroxide spin-labeled netropsin-acridine hybrid in living KB cells: Electron Spin

C Bailly1, J P Hénichart

  • 1INSERM U16, Lille, France.

Biochemical and Biophysical Research Communications
|March 16, 1990
PubMed

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...