Jove
Visualize
Contact Us

Related Concept Videos

Biofuels01:25

Biofuels

The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...
Tissue Homogenization and Cell Lysis01:32

Tissue Homogenization and Cell Lysis

Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pharmacokinetics and molecular-level insights into 5-Methyl-3-(trifluoromethyl)-1H-pyrazole for anticancer action: Spectroscopic profiling, solvent interactions, topological analysis and ADME-QSAR predictions.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2025
Same author

A prospective observational study on changes in endo-tracheal tube cuff pressure and its correlation with airway pressures during various stages of robotic pelvic surgeries.

Journal of anaesthesiology, clinical pharmacology·2022
Same author

Efficacy of marine cyanobacterium Oxynema thaianum ALU PBC5 against multi drug resistant Gram negative pathogens.

Journal of applied microbiology·2020
Same author

Anaesthetic management and considerations in a patient with chronic dicyclomine addiction.

Indian journal of anaesthesia·2020
Same author

Thermodynamic databases for proteins and protein-nucleic acid interactions.

Biopolymers·2002
Same author

Thermodynamic database for protein-nucleic acid interactions (ProNIT).

Bioinformatics (Oxford, England)·2001
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 2, 2026

Analysis of Fatty Acid Content and Composition in Microalgae
07:44

Analysis of Fatty Acid Content and Composition in Microalgae

Published on: October 1, 2013

A comparative study on effective cell disruption methods for lipid extraction from microalgae.

P Prabakaran1, A D Ravindran

  • 1Department of Biology, Gandhigram Rural Institute-Deemed University, Gandhigram, Tamilnadu, India.

Letters in Applied Microbiology
|May 18, 2011
PubMed
Summary

Sonication is the most effective method for disrupting microalgae cells to extract lipids for biodiesel. This method yielded the highest lipid content from Chlorella sp. microalgae.

More Related Videos

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
11:08

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

Published on: January 7, 2019

A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii
07:51

A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii

Published on: August 8, 2019

Related Experiment Videos

Last Updated: Jun 2, 2026

Analysis of Fatty Acid Content and Composition in Microalgae
07:44

Analysis of Fatty Acid Content and Composition in Microalgae

Published on: October 1, 2013

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
11:08

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

Published on: January 7, 2019

A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii
07:51

A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii

Published on: August 8, 2019

Area of Science:

  • Biotechnology
  • Microalgal lipid extraction
  • Biodiesel production

Background:

  • Microalgae are a sustainable source for biofuel production.
  • Efficient lipid extraction is critical for economic viability.
  • Cell disruption is a key step in lipid extraction.

Purpose of the Study:

  • To compare the effectiveness of different cell disruption methods for lipid extraction from freshwater microalgae.
  • To identify the optimal method for maximizing lipid yield.
  • To analyze the fatty acid composition of extracted lipids.

Main Methods:

  • Microalgae species (Chlorella sp., Nostoc sp., Tolypothrix sp.) were isolated and cultured.
  • Cell disruption was performed using autoclaving, bead beating, microwave, sonication, and 10% NaCl solution.
  • Lipid extraction was conducted using a chloroform-methanol mixture.
  • Fatty acid composition was analyzed using gas chromatography (GC).

Main Results:

  • Sonication proved to be the most effective cell disruption method.
  • Chlorella sp. yielded the highest lipid content.
  • Nostoc sp. and Tolypothrix sp. showed high oleic acid content (13.27 mg g(-1) dw and 17.75 mg g(-1) dw, respectively).
  • Chlorella sp. exhibited high linoleic acid content (17.61 mg g(-1) dw) when sonication was applied.

Conclusions:

  • Sonication is a highly applicable and efficient method for microalgal lipid extraction.
  • Optimizing cell disruption techniques is crucial for enhancing lipid extraction efficiency in biodiesel production.