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Updated: Jun 2, 2026
Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
Multiplex allele-specific polymerase chain reaction for detection of isoniazid resistance in Mycobacterium
D Q Tho1, N T N Lan, N V V Chau
1University of Oxford Clinical Research Unit, Wellcome Trust Major Overseas Programme, Hospital for Tropical Diseases, Ho Chi Minh City, Viet Nam.
Setting:
Pham Ngoc Thach Tuberculosis Reference Hospital, Ho Chi Minh City, Viet Nam.
Design:
A multiplex allele-specific polymerase chain reaction (MAS-PCR) was developed to detect mutations at the two most common sites responsible for isoniazid (INH) resistance in Mycobacterium tuberculosis: katG315 and inhA-15. The MAS-PCR is able to detect rare mutations at katG315, in addition to katG S315T. Conventional phenotypic proportion drug susceptibility testing on Löwenstein-Jensen media was used as a gold standard to compare the sensitivity and specificity of the commercial MTBDRplus line-probe assay and the MAS-PCR in 100 INH-resistant and 50 INH-susceptible isolates collected consecutively at Pham Ngoc Thach Hospital reference laboratory.
Results:
The sensitivity and specificity on culture isolates were 90% (n = 90/100, 95%CI 0.83-0.94) and 100% (n = 50/50, 95%CI 0.93-1.0), respectively, for the MAS-PCR and the MTBDRplus assay.
Conclusion:
The MAS-PCR described here represents an alternative method for rapid screening for INH resistance in M. tuberculosis isolates.
