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Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
10:24

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

Published on: October 28, 2014

[Changes in RBC Ca2(+)-ATPase activity and red-cell calcium concentration during dialysis in patients with uremia]

Y Yang

    Zhonghua Yi Xue Za Zhi
    |January 1, 1990
    PubMed

    Insights

    Uremia patients undergoing dialysis showed decreased red blood cell (RBC) Ca2(+)-ATPase activity and increased calcium levels. Hemodialysis and continuous ambulatory peritoneal dialysis (CAPD) partially improved these RBC parameters.

    Area of Science:

    • Nephrology
    • Biochemistry
    • Cell Physiology

    Background:

    • Uremia is associated with altered cellular ion transport.
    • Red blood cell (RBC) calcium homeostasis may be disrupted in uremic patients.

    Purpose of the Study:

    • To investigate the impact of dialysis on RBC Ca2(+)-ATPase activity and intracellular calcium concentration in uremic patients.

    Main Methods:

    • Observational study involving 19 uremic patients undergoing either hemodialysis or CAPD.
    • Measurement of RBC Ca2(+)-ATPase activity and red cell calcium concentration before and after dialysis treatments.

    Main Results:

    • Hemodialysis patients exhibited significantly lower RBC Ca2(+)-ATPase activity and higher red cell calcium compared to controls.
    • Hemodialysis led to a significant increase in Ca2(+)-ATPase activity and a decrease in red cell calcium.
    • CAPD patients also showed reduced RBC Ca2(+)-ATPase activity and elevated red cell calcium.
    • One month of CAPD resulted in a slight increase in Ca2(+)-ATPase activity and a significant reduction in red cell calcium.

    Conclusions:

    • Dialysis significantly impacts RBC calcium regulation in uremic patients.
    • Hemodialysis appears to offer a more immediate improvement in RBC calcium handling compared to CAPD.
    • Further research is needed to fully elucidate the long-term effects of different dialysis modalities on RBC function in uremia.

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