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Published on: July 30, 2018
[Construction and confirmation of a recombinant adenovirus vector of survivin]
Yuan-Jing Lü1, Su-Sheng Miao, Wei-Song Liu
1Department of Head and Neck Surgery, The Third Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
Objective:
To construct a recombinant adenovirus of survivin vector and provid valuable reference for gene therapy of laryngeal cancer.
Methods:
The survivin gene was cloned by PCR. After confirmation by enzyme restriction analysis and sequencing, the gene and the adenovirus vector were recombined together to construct the recombinant adenovirus vector. The recombinant adenovirus vector was confirmed via both sequencing and digestion restriction analysis, and then linearized and transfected into the HEK 293 cell line to generate recombinant adenovirus.
Results:
The sequence analysis demonstrated that the survivin gene sequence was the same as published in the literature, suggesting that a recombinant adenovirus vector has been successfully constructed.
Conclusions:
A survivin recombinant adenovirus has been successfully constructed.
