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Updated: Jun 2, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
Published on: March 22, 2018
Chromosomal position effects are linked to sir2-mediated variation in transcriptional burst size
Cory Batenchuk1, Simon St-Pierre, Lioudmila Tepliakova
1Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, Ontario, Canada.
Abstract:
Gene expression noise varies with genomic position and is a driving force in the evolution of chromosome organization. Nevertheless, position effects remain poorly characterized. Here, we present a systematic analysis of chromosomal position effects by characterizing single-cell gene expression from euchromatic positions spanning the length of a eukaryotic chromosome. We demonstrate that position affects gene expression by modulating the size of transcriptional bursts, rather than their frequency, and that the histone deacetylase Sir2 plays a role in this process across the chromosome.
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