Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
Autophagic Cell Death01:18

Autophagic Cell Death

Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and pro-apoptotic...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tetanus mimicking post-radiotherapy dysphagia in a head and neck cancer survivor:A case report with institutional review.

Auris, nasus, larynx·2026
Same author

Recurrent laryngeal nerve schwannoma adjacent to the thyroid: a case report.

Journal of medical case reports·2026
Same author

A Rare Case of Hemangioma in the Thyroid: Clinical Presentation and Management Insights.

The Tokai journal of experimental and clinical medicine·2025
Same author

Shigella type-III secretion system effectors counteract the induction of host inflammation and cell death.

The EMBO journal·2025
Same author

Evaluating Clinical Outcomes and Predictive Factors of Treatment Approaches in T1-3N3b Hypopharyngeal Carcinoma: Upfront Neck Dissection Versus Concurrent Chemoradiotherapy.

Head & neck·2025
Same author

A Case of Ectopic Thyroid Cancer in the Abdominal Cavity.

The Tokai journal of experimental and clinical medicine·2025

Related Experiment Video

Updated: Jun 2, 2026

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination
07:58

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination

Published on: January 2, 2026

A Tecpr1-dependent selective autophagy pathway targets bacterial pathogens.

Michinaga Ogawa1, Yuko Yoshikawa, Taira Kobayashi

  • 1Division of Bacterial Infection, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. ogacho@ims.u-tokyo.ac.jp

Cell Host & Microbe
|May 18, 2011
PubMed
Summary

Researchers discovered Tecpr1, a protein crucial for selective autophagy, which targets bacterial pathogens for degradation. This pathway is vital for innate immunity and preventing pathogen multiplication within host cells.

More Related Videos

Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization
09:25

Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization

Published on: July 28, 2015

Related Experiment Videos

Last Updated: Jun 2, 2026

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination
07:58

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination

Published on: January 2, 2026

Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization
09:25

Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization

Published on: July 28, 2015

Area of Science:

  • Cell Biology
  • Immunology
  • Molecular Biology

Background:

  • Selective autophagy is a host defense mechanism targeting bacterial pathogens.
  • Mechanisms for targeting specific cargo receptors in autophagy remain unclear.

Purpose of the Study:

  • To identify novel proteins involved in the selective autophagy pathway.
  • To elucidate the role of Tecpr1 in targeting bacterial pathogens for autophagic degradation.

Main Methods:

  • Co-immunoprecipitation to identify Atg5 binding partners.
  • Confocal microscopy to assess colocalization of Tecpr1 with Atg5 and WIPI-2.
  • Analysis of Tecpr1-deficient mouse embryonic fibroblasts (MEFs) for autophagic defects and bacterial load.
  • Assessment of mitochondrial and protein aggregate accumulation in Tecpr1-knockout MEFs.

Main Results:

  • Tecpr1 was identified as an Atg5 binding partner colocalizing with Atg5 at Shigella-containing phagophores.
  • Tecpr1 is essential for efficient autophagic targeting of bacteria but not canonical autophagy.
  • Tecpr1 interacts with and colocalizes with WIPI-2 on phagophores.
  • Tecpr1-deficient MEFs showed impaired selective autophagy, increased intracellular Shigella multiplication, and accumulation of depolarized mitochondria and protein aggregates.

Conclusions:

  • Tecpr1 is a key component of a novel pathway for targeting bacterial pathogens via selective autophagy.
  • This Tecpr1-dependent pathway plays a significant role in innate immunity against bacterial infections.
  • Tecpr1 deficiency leads to cellular defects associated with impaired selective autophagy.