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Regulated expression of Epstein-Barr virus nuclear antigen 3-encoding gene carried on stable episomal vectors in
M R James1, A R Sarasin, M Perricaudet
1Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Abstract:
Recently a small number of Epstein-Barr viral (EBV) genes, characteristically expressed in latently infected, growth-transformed B-lymphocytes, have been cloned and several have been transiently expressed by DNA transfection. Here we demonstrate production of stable human cell lines containing episomal EBV vectors and expressing EBV nuclear antigen 3 from the adenovirus major late promoter or the mouse metallothionein promoter, which retains metal-regulation in the episomal state. This system has proved useful in an analysis of the role of these and other EBV genes implicated in immortalization and/or oncogenic transformation of human cells.