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Related Experiment Video

Updated: Jun 2, 2026

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

Published on: May 5, 2012

HIV-1 remodels the nuclear pore complex.

Anne Monette¹, Nelly Panté, Andrew J Mouland

¹HIV-1 RNA Trafficking Laboratory, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Montréal, Québec H3T 1E2, Canada.

The Journal of Cell Biology

|May 18, 2011

Summary

Human immunodeficiency virus type 1 (HIV-1) disrupts the nuclear envelope (NE) by altering nucleoporin (Nup) levels and localization. Nup62 is crucial for HIV-1 replication and infectivity.

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Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

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Published on: May 5, 2012

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Published on: June 26, 2019

Area of Science:

Cell Biology
Virology
Molecular Biology

Background:

Human immunodeficiency virus type 1 (HIV-1) hijacks host cell machinery for replication.
Previous studies indicated HIV-1 causes cytoplasmic retention of RNA-binding proteins.
This retention is linked to viral genomic RNA export and nucleoporin p62 (Nup62) alterations.

Purpose of the Study:

To investigate the comprehensive impact of HIV-1 infection on the nuclear envelope (NE) proteome.
To elucidate the role of nucleoporins (Nups) in HIV-1 replication.

Main Methods:

Proteomics analysis of nuclear envelopes from HIV-1 infected T cells.
Immunogold electron microscopy to visualize Nup translocation.
Small interfering RNA (siRNA) depletion to assess Nup62 function.

Main Results:

HIV-1 infection significantly altered NE composition, decreasing Nup abundance.
Nups were observed translocating into the cytoplasm.
Nup62 was found in purified HIV-1 particles and is essential for viral gene expression and infectivity.

Conclusions:

HIV-1 infection profoundly affects NE composition and organization.
Viral manipulation of Nups, particularly Nup62, is critical for the virus's life cycle.
This highlights the extensive viral control over host cell biology.