A reliable method for establishing viral infection in the rabbit by intranasal inoculation

Bovine herpesvirus 1 (BHV-1) infection was established in the rabbit by a novel procedure which involved virus being injected through trephine openings on either side of the nose of anaesthetised animals. This method of intranasal inoculation produced more consistent reactions to BHV-1 inoculation, and both humoral and cell-mediated immune responses to the virus were of greater magnitude compared with those following per naris instillation of virus.