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Rotavirus serotypes 6 and 10 predominate in cattle
D R Snodgrass1, T Fitzgerald, I Campbell
1Moredun Research Institute, Edinburgh, Scotland.
Journal of Clinical Microbiology
|March 1, 1990
Summary
Calf fecal rotavirus strains were serotyped, with serotype 6 being the most common. Novel bovine rotavirus strains were identified, establishing serotype 10 as a major type.
Area of Science:
- Veterinary Virology
- Microbiology
- Immunology
Background:
- Bovine rotaviruses are a significant cause of calf diarrhea.
- Accurate serotyping is crucial for understanding rotavirus epidemiology and vaccine development.
Purpose of the Study:
- To serotype calf fecal rotavirus strains.
- To identify and characterize novel bovine rotavirus serotypes.
Main Methods:
- Enzyme-linked immunosorbent assays (ELISAs) using monoclonal antibodies against VP7 and VP4 proteins.
- Adaptation of untyped strains to cell culture.
- Cross-neutralization tests.
Main Results:
- Sixty-six percent of 162 samples were typed as serotype 6, and 7% as serotype 10.
- Untyped strains often lacked sufficient virus or represented novel subtypes.
- A distinct group of bovine rotaviruses, including B223, justified the establishment of serotype 10.
Conclusions:
- Serotype 6 is the predominant bovine rotavirus serotype.
- Serotype 10 represents a distinct and significant bovine rotavirus type.
- Further characterization of bovine rotavirus diversity is warranted.