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Updated: Jun 1, 2026
10:33
Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
Published on: October 15, 2018
N,3'-cyclo-5'-O-Cyano-methyl-thymidine
Jingbo Sun1, Kun Yang, Ronghui Duan
1College of Chemistry, Jilin University, Changchun 130012, People's Republic of China.
Summary
This study details a novel cyclo-nucleoside with a C-N linkage. Structural analysis reveals its specific furan-ose ring conformation and hydrogen bonding patterns, important for understanding nucleoside analogs.
Area of Science:
- Medicinal Chemistry
- Structural Biology
- Crystallography
Background:
- Cyclo-nucleosides are important pharmaceutical compounds.
- Understanding their structure is key to drug design.
Purpose of the Study:
- To characterize the structure of a novel cyclo-nucleoside.
- To analyze its conformation and intermolecular interactions.
Main Methods:
- X-ray crystallography was used to determine the molecular structure.
- Conformational analysis of the furan-ose ring was performed.
Main Results:
- The compound is a cyclo-nucleoside with a C-N linkage (C19H20N4O4).
- The furan-ose ring adopted a twist C3'-endo/C2'-exo conformation (P=8.1°, vm=30.6°).
- The pyrimidine ring was in an anti orientation, with intra-molecular and inter-molecular hydrogen bonds observed.
Conclusions:
- The determined structure provides a basis for understanding the properties of this cyclo-nucleoside.
- The observed conformation and hydrogen bonding are critical for its potential biological activity.
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