Developmentally regulated changes of glutamate binding sites in mouse deep cerebellar nuclei

The expression of L-[3H]glutamate binding sites of different ionic and pharmacological sensitivities was studied in mouse deep cerebellar nuclei during early postnatal development by means of in vitro autoradiography. Ca2+/Cl(-)-dependent, quisqualate/AMPA/ibotenate-sensitive, and APB-insensitive binding sites are present at high density in the deep cerebellar nuclei of young animals, but greatly decrease between the 10th and 25th postnatal day and remain low in the adult. The density of Ca2+/Cl(-)-independent binding sites remains low and constant during the whole of postnatal development. The possible involvement of the Ca2+/Cl(-)-dependent binding sites in brain development is discussed.