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Updated: Jun 1, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
Published on: November 23, 2016
2-Hydr-oxy-3-nitro-benzamide
Abstract:
The asymmetric unit of title compound, C(7)H(6)N(2)O(4), contains two mol-ecules, one of which has a disordered nitro group with an occupancy ratio of 0.517 (9):0.483 (9) for the O atoms. Both mol-ecules contain an intra-molecular O-H⋯O hydrogen bond. In the crystal, both mol-ecules form inversion dimers linked by pairs of N-H⋯O hydrogen bonds, resulting in R(2) (2)(8) ring motifs. The dimers are connected by further N-H⋯O links and weak C-H⋯O inter-actions, resulting in a layered motif.
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The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by water loss...
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