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Updated: Jun 1, 2026
09:26
Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
3α-Hydr-oxy-ent-atis-16-en-14-one
Summary
Researchers isolated an ent-atisane diterpenoid from Euphorbia kansuensis roots. Its crystal structure reveals complex ring conformations and intermolecular hydrogen bonding, forming zigzag chains.
Area of Science:
- Natural Product Chemistry
- Organic Chemistry
- Crystallography
Background:
- Euphorbia kansuensis is a plant species known to produce various bioactive compounds.
- Diterpenoids, particularly ent-atisane types, are a significant class of natural products with diverse chemical structures and biological activities.
- Understanding the structural and conformational properties of isolated natural products is crucial for elucidating their function and potential applications.
Purpose of the Study:
- To isolate and characterize a specific ent-atisane diterpenoid from Euphorbia kansuensis.
- To determine the molecular structure and conformational analysis of the isolated compound.
- To investigate the crystal structure and intermolecular interactions of the diterpenoid.
Main Methods:
- Isolation of the title compound from the roots of Euphorbia kansuensis.
- Determination of the molecular formula as C(20)H(30)O(2).
- Analysis of the crystal structure using X-ray diffraction to elucidate molecular conformation and packing.
Main Results:
- An ent-atisane diterpenoid was successfully isolated.
- The molecule features five six-membered rings: three in boat conformations within a bicyclo-[2.2.2]octane unit and two cyclohexane rings in chair conformations.
- The crystal structure exhibits intermolecular O-H⋯O hydrogen bonds, leading to the formation of zigzag chains oriented parallel to the [001] crystallographic direction.
Conclusions:
- The study successfully characterized a novel ent-atisane diterpenoid from Euphorbia kansuensis.
- The detailed structural analysis provides insights into the conformational flexibility of this diterpenoid class.
- The observed hydrogen bonding and chain formation in the crystal lattice offer valuable information for understanding solid-state properties and potential intermolecular interactions.
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