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Updated: Jun 1, 2026
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Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
Published on: July 1, 2018
[Instantaneous expression aFGF-GFP fusion gene in safflower]
Jing Yang1, Yongxin Guo, Suping Ren
1Jilin Agricultural University, Ministry of Education Bioreactor and Drug Development Research Center, Jilin 130118, China. xiziyang512@163.com
Summary
Researchers successfully expressed the acidic fibroblast growth factor (aFGF)-green fluorescent protein (GFP) fusion gene in safflower (Carthamus tinctorius) calli. This demonstrates the successful expression of the aFGF gene in plant cells.
Area of Science:
- Molecular Biology
- Plant Biotechnology
- Gene Expression Analysis
Context:
- Investigating gene expression in plants is crucial for crop improvement and understanding plant development.
- Acidic fibroblast growth factor (aFGF) plays a role in cell growth and differentiation.
- Safflower (Carthamus tinctorius) is an important oilseed crop with potential for genetic modification.
Purpose:
- To study the instantaneous expression of the aFGF-GFP fusion gene in Carthamus tinctorius.
- To establish a method for transforming safflower with foreign genes.
- To visualize and confirm the expression of the aFGF-GFP fusion gene in plant cells.
Summary:
- A plant expression vector, pCAMBIA1390::35S::aFGF-GFP, was constructed by fusing the aFGF and GFP genes.
- This vector was transformed into Carthamus tinctorius using Agrobacterium tumefaciens-mediated transformation.
- Transformed safflower calli exhibited strong green fluorescence under UV light, confirmed by fluorescence microscopy and PCR amplification of both genes.
Impact:
- This study successfully demonstrates the expression of the aFGF-GFP fusion gene in safflower.
- The findings provide a foundation for further research into the function of aFGF in safflower.
- The established transformation protocol can be applied to introduce other genes into Carthamus tinctorius for crop improvement.
