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[Classification of Pueraria lobata in different geographical regions]
Dongji Liu1, Zhikui Yu, Chunsheng Liu
1School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. year_365@163.com
Objective:
To classify Pueraria lobata originated from different geographical regions based on ITS,psbK-psbI and trnH-psbA information.
Method:
Twenty-four samples of P. lobata were collected from northeast China, north China, central China and northwest China. DNA extraction, PCR, sequence and genotypes/haplotypes analysis were performed .
Result:
ITS1, 5.8S and ITS2 varied only 1 bp respectively, psbK-psbI 2 bps; trnH-psbA varied 1 bp and 10 bp deletion.
Conclusion:
Based on the variation of ITS,psbK-psbI and trnH-psbA, 4 genotypes and 2 haplotypes were identified, respectively.
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