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Published on: February 20, 2012
[AFLP analysis of genetic diversity of Alpinia officinarum]
Quan Yang1, Chunrong Zhang, Hubiao Chen
1School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China. yangquan7208@vip.163.com
Objective:
To explore the genetic diversity and relationship of different Alpinia officinarum germplasm.
Method:
Amplified fragment length polymorphism (AFLP) markers were developed to analyze genetic polymorphism in A. officinarun from eight resources. The amplified fragments were used as primary matrix with NTSYSpc-2.11F software to analyze the similarity between the A. officinarum germplasm and to construct the genetic phylogenetic tree.
Result:
A total of 1,120 fragments were genotyped using AFLP with eight prime combinations. Analysis identified 1,044 polymorphic fragments, accounting for 92.57% of the total detected variation. Genetic phylogenetic tree analysis indicates that three categories can be divided among the eight resources of A. officinanrum.
Conclusion:
Significant polymorphism and genetic diversity can be observed among A. officinarum germplasm resources.
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