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Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
Published on: June 23, 2012
[Analysis of genetic diversity of gemplasm of Pinellia ternata based on SRAP]
1Hubei College of Traditional Chinese Medicine, Key Laboratory of Traditional Chinese Medicine Resource and Traditional Chinese Medicine Compound Co-constructed by Hubei Province and Ministry of Education, Wuhan 430065, China. ym-e@tom.com
Objective:
To assess population genetic diversity of Pinellia ternata with different phenotype and from different habitat by sequence-related amplified polymorphism (SRAP) technique.
Method:
Fourteen appropriate primer pairs were selected out from a total of 80 pairs for SRAP PCR amplification. A Jaccard's genetic similarity matrix and a dendrogram were established using SPSS 16.0 software.
Result:
The 14 primer pairs could amplify 171 bands, and the percentage of polymorphic bands was 15.8%. Based on the dendrogram, P. ternata in the same habitat clustered in a clade.
Conclusion:
These results suggested that SRAP markers could be used as an effective molecular technique for the diversity study of P. ternata and the habitat was more important than the phenotype in identification of P. ternata germplasm resource.
