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Updated: Jun 1, 2026

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Development and characterization of 12 microsatellite markers from the deep-sea hydrothermal vent siboglinid Riftia
A J Fusaro1, A R Baco, G Gerlach
1Biology Department, MS#33, Redfield Laboratory, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543, USA.
Abstract:
Ecological processes at deep-sea hydrothermal vents on fast-spreading mid-ocean ridges are punctuated by frequent physical disturbance, often accompanied by a high occurrence of population turnover. To persist through local extinction events, sessile invertebrate species living in these geologically and chemically dynamic habitats depend on larval dispersal. We characterized 12 polymorphic microsatellite loci from one such species, the siboglinid tubeworm Riftia pachyptila. All loci conformed to Hardy-Weinberg expectations without linkage (mean H(E) = 0.9405, mean N(A) = 20.25). These microsatellites are being employed in the investigation of spatial and temporal population genetic structure in the eastern Pacific Ocean.
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