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Updated: Jun 1, 2026

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Development of 16 microsatellite markers for the European cockchafer, Melolontha melolontha
Jürg Enkerli1, Alexandra Gisler, Roland Kölliker
1Agroscope Reckenholz-Tänikon Research Station, Molecular Ecology, Reckenholzstrasse 191, 8046 Zürich, Switzerland.
Abstract:
Sixteen polymorphic microsatellite markers were isolated from the European cockchafer, Melolontha melolontha L., based on enriched genomic libraries. Allelic variability was assessed in a collection of 30 individuals obtained from a field in eastern Switzerland. The number of detected alleles ranged from three to 13 with a mean of 6.69 and average observed and expected heterozygosities were 0.52 and 0.66, respectively. The 16 highly polymorphic microsatellite markers represent a powerful molecular tool, which will allow for detailed population genetic analyses on this important pest insect.

