Related Experiment Video
Updated: Jun 1, 2026
High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay
Published on: March 10, 2020
PERMANENT GENETIC RESOURCES: Development of microsatellite markers for the guava rust fungus, Puccinia psidii
Shaobin Zhong1, Baojun Yang, Acelino Couto Alfenas
1Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, HI 96822, USA, Department of Plant Pathology, North Dakota State University, Fargo, ND 58105, USA, Departamento de Fitopatologia, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brazil.
Abstract:
We developed and characterized 15 polymorphic microsatellite markers present in the genome of the guava rust fungus, Puccinia psidii. The primers for these microsatellite markers were designed by sequencing clones from a genomic DNA library enriched for a simple sequence repeat (SSR) motif of (AG). All these 15 primer pairs successfully amplified DNA fragments from a sample of 22 P. psidii isolates, revealing a total of 71 alleles. The observed heterozygosity at the 15 loci ranged from 0.05 to 1.00. The SSR markers developed would be useful for population genetics study of the rust fungus.
