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Updated: Jun 1, 2026
A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
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PERMANENT GENETIC RESOURCES: Isolation, characterization and cross-species amplifications of microsatellite loci from
C E Edwards1, D E Soltis, P S Soltis
1Department of Botany, University of Florida, Gainesville, FL 32611, USA, Florida Museum of Natural History, University of Florida, Gainesville, FL 32611, USA.
Abstract:
We report the isolation of microsatellite loci from three species in the genus Conradina (Lamiaceae). To ensure their utility for multiple species, loci were screened for amplification and variability in all six Conradina species; 11 loci demonstrated high levels of amplification and polymorphism in most species. These 11 loci were characterized in 20 individuals from one population of Conradina brevifolia; alleles per locus ranged from five to 15, and observed heterozygosity ranged from 0.30 to 0.90. These microsatellites will be used to clarify species limits, detect interspecific hybridization, and understand the partitioning of genetic variation in each species of Conradina.
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