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Isolation and characterization of microsatellite markers in black muntjac (Muntiacus crinifrons)
Hai-Long Wu1, Kai Meng, Guo-Ping Zhu
1College of Life Science, the Key Laboratory of Biotic Environment and Ecological safety in Anhui Province and the Key Laboratory of Molecular Evolution and Biodiversity, Anhui Normal University, Wuhu 241000, China.
Abstract:
The black muntjac (Muntiacus crinifrons) is a vulnerable species found in the mountains of eastern China, about which little is known. Here we develop 11 polymorphic microsatellite loci from a (CA)(n) -enrichment library for the animal. In the analyses of 25 individuals sampled, unbiased expected heterozygosity levels varied from 0.686 to 0.876 and the number of alleles per locus varied from five to nine. Results that 11 microsatellite loci were highly polymorphic indicated that these markers are sufficiently powerful to address such questions as parentage, mating system and population genetic structure of M. crinifrons.
