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Fluorescence-Activated Cell Sorting for the Isolation of Scleractinian Cell Populations
Published on: May 31, 2020
Microsatellite loci isolated from the scleractinian coral, Acropora nobilis
1Graduate School of Engineering and Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan, Department of Chemistry, Biology and Marine Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan.
Abstract:
We report the isolation and characterization of eight microsatellite loci from the scleractinian coral, Acropora nobilis. The microsatellite loci were obtained using compound SSR primers or an enrichment protocol. All the loci were polymorphic with four to eight alleles per locus and observed heterozygosities ranging from 0.22 to 0.76. Some of the primers developed for the two congeners, Acropora palmata and Acropora millepora were applicable to A. nobilis. These loci are useful for studying the connectivity among A. nobilis populations in Okinawa, southern Japan.
