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Identification and characterization of simple sequence repeat markers from a glandular Origanum vulgare expressed
Johannes Novak1, Brigitte Lukas, Kerstin Bolzer
1Institute for Applied Botany, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria, Department of Biochemistry, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, D-07745 Jena, Germany.
Abstract:
Oregano (Origanum vulgare) and marjoram (Origanum majorana) are two sensorial distinct spices within the genus Origanum (Lamiaceae). Simple sequence repeat (SSR) markers were developed from expressed sequence tags (ESTs) of essential oil glands of O. vulgare. Thirteen EST-SSR loci were evaluated using 20 individual plants of O. vulgare and 19 plants of Origanum majorana. The number of alleles per locus ranged from one to four. All loci developed from O. vulgare successfully cross-amplified in O. majorana.
