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Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Eleven polymorphic microsatellite markers in Cory's shearwater, Calonectris diomedea, and cross-species amplification
Joël Bried1, Marie-Pierre Dubois, Pierre Jouventin
1Departamento de Oceanografia e Pescas, Centro do IMAR da Universidade dos Açores, 9901-862 Horta, Açores, Portugal, CEFE, UMR 5175 CNRS, 1919 route de Mende, 34293 Montpellier cedex 5, France.
Abstract:
For the first time, we describe 11 variable dinucleotide microsatellites and the conditions for multiplexing and simultaneous genotyping sets of loci in Cory's shearwater, Calonectris diomedea. Microsatellite variability was assessed in a colony from the Azores archipelago (Atlantic Ocean). Two to eight alleles were detected per locus, the mean gene diversity being 4.5. Cross-species amplification in three other seabirds (Diomedea exulans, Procellaria aequinoctialis and Bulweria bulwerii) revealed some variability at one, two and eight loci, respectively.
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