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Published on: October 5, 2017
Isolation and cross-amplification of microsatellites in pink abalone (Haliotis corrugata)
N Díaz-Viloria1, R Pérez-Enríquez, G Fiore-Amaral
1Aquaculture Genetics Laboratory, Centro de Investigaciones Biológicas del Noroeste S.C., Mar Bermejo 195 Col. Playa Palo de Santa Rita, La Paz, B.C.S. 23090, México, Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0202, USA.
Abstract:
Ten novel microsatellite loci were isolated in pink abalone, Haliotis corrugata, using (GT)(15) and (CT)(15 ) enriched genomic libraries. Two previously reported Haliotis kamtschatkana microsatellites cross-amplified in H. corrugata. A set of 12 polymorphic microsatellites were evaluated in a wild population sample (N = 49). The number of alleles ranged from two to 55, and the observed and expected heterozygosities ranged from 0.104 to 0.939 and from 0.213 to 0.982, respectively. Significant deviations from Hardy-Weinberg equilibrium at three loci and no linkage disequilibrium were observed. Haliotis corrugata microsatellites cross-amplified in other abalone species, two in H. fulgens, and seven in H. rufescens.
