Characterization of 21 microsatellites developed from Propithecus deckeni deckeni
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Published on: July 26, 2010
Runhua Lei1, Sabine Day, Roger Ramarokoto
1Center for Conservation and Research, Henry Doorly Zoo, 3701 South 10th Street, Omaha, NE 68107, USA, Department of Palaeontology and Anthropology, University of Antananarivo, Madagascar.
Abstract:
Decken's sifaka (Propithecus deckeni deckeni) is found between the Mahavavy and Manambolo Rivers, in western Madagascar. Twenty-one polymorphic nuclear microsatellite loci were isolated from genomic DNA derived from a P. d. deckeni, from Tsingy de Bemaraha National Park. Population genetic parameters were estimated on 10 individuals each, sampled from Tsingy de Bemaraha National Park and Tsiombikibo Classified Forests. Significant null alleles were detected in seven loci which were dropped before population genetic parameters were re-estimated and compared to the full marker suite.
